Calvin C. Hale, PhD

Email: HaleC@missouri.edu

Research Interests: Cardiac membrane transport

Teaching: Cell biology

Dr. Hale's research attempts to understand on a molecular level how membrane transport proteins function.

His major interest is the cardiac sodium-calcium exchanger, an important regulator of calcium homeostasis, and thus contractility, during diastole. The sodium-calcium exchange protein is a target for the development of novel inotropic agents for the treatment of congestive heart failure. Dr. Hale has identified a regulatory region on the sodium-calcium exchange protein that, when occupied by an exogenous ligand, can inhibit the exchange process. The regulatory region consists of highly charged, acidic amino acids situated on a large internal loop of the exchanger protein. His current work attempts to topologically map and define this region for the purpose of drug design.

Publications:
Hale, C.C., Hill, C.K., Price, E.M., and J. Bossuyt, 2002, Expressing and purifying membrane transport proteins in high yield. J. Biochem. Biophys. Meth. 50: 233-243.

Bossuyt, J., James-Kracke, M., and C.C. Hale, 2002, The cardiac sodium-calcium exchanger is associated with caveolin-3. FEBS Let. 511: 113-117.

Bossuyt, J., Taylor, B.E., James-Kracke, M., and C.C. Hale, 2002, The cardiac sodium-calcium exchanger associates with caveolin-3. NY Acad. Sci. (in press).

Hale, C.C., Bossuyt, J., Hill, C.K., Price, E.M., Schulze, D.H., Lederer, W.J., Poljak, R., and B.C. Braden, 2002, Sodium-calcium exchange crystallization. NY Acad. Sci. (in press).

Hale, C.C., Hill, C.K., Price, E.M., and J. Bossuyt, 2002, Expressing and purifying membrane transport proteins in high yield. J. Biochem. Biophys. Meth. 50: 233-243.

Bossuyt, J., James-Kracke, M., and C.C. Hale, 2002, The cardiac sodium-calcium exchanger is associated with caveolin-3. FEBS Let. 511: 113-117.

Gatto C, Hale CC, Xu W, and Milanick MA. Eosin, a potent inhibitor of the plasma membrane Ca pump, does not inhibit the cardiac Na-Ca exchanger. Biochemistry, 34:965-972, 1995.

Dominguez JH, Hale CC, and Qulali M. Studies of Renal Injury I. Gentamicin toxicity and the expression of basolateral transporters. Am. J. Physiol. 270: F245-F253, 1996.

Hale CC. Mechanism of XIP in cardiac sarcolemmal vesicles. Proceedings of the Third International Conference on Sodium-Calcium Exchange, Annals of the New York Academy of Sciences, 779:171-181, 1996.

Gatto C, Xu W-Y, Denison HA, Hale CC, and Milanick MA. Modifications of XIP, the autoregulatory region of the Na/Ca exchanger and its ability to inhibit the Na/Ca exchanger in bovine sarcolemmal vesicles. Proceedings of the Third International Conference on Sodium-Calcium Exchange, Annals of the New York Academy of Sciences, 779:284-285, 1996.

Ford DA, and Hale CC. Plasmalogen and anionic phospholipid dependence of the cardiac sodium-calcium exchanger. FEBS Letters 394:99-102, 1996.

Xu W, Denison H, Hale CC, Gatto C, and Milanick MA. Identification of critical positive charges in XIP, the Na/Ca exchange inhibitory peptide. Arch. Biochem. Biophys. 341:273-279, 1997.

Hale CC, Bliler S, Quinn TP, and Peletskaya EN. Localization of the exchange inhibitory peptide (XIP) binding site on the cardiac sodium-calcium exchanger. Biochem. Biophys. Res. Comm. 236:113-117, 1997.

Hale CC, Ebeling EG, Hsu FF, and Ford DA. The selective activation of the cardiac sarcolemmal sodium-calcium exchanger by plasmalogenic phosphatidic acid produced by phospholipase D. FEBS Letters 422:247-251, 1998.

Hale CC, Zimmerschied JA, Bliler S, and Price EM. Large-scale expression of recombinant cardiac sodium-calcium exchange in insect larvae. Protein Expression and Purification. 15:121-126, 1999.